Now in its sixth edition, Principles of Gene Manipulation provides an excellent introduction to the area of genetic engineering of plants, animals and...
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Now in its sixth edition, Principles of Gene Manipulation provides an excellent introduction to the area of genetic engineering of plants, animals and microbes for advanced level undergraduates with a basic understanding of genetics. This classic textbook has been substantially updated and revised to reflect the rapid advances that have been made in the core technologies in the seven years since the last edition. Furthermore, to put theses technologies into context, the final chapter has been strucred into six themes : nucleid acids as diagnostic tools, new drugs and new therapies for genetic diseases, combating infectious disease, protein engineering, metabolic engineering, modern plant breeding.
Sommaire
Gene manipulation : an all-embracing technique
Basic techniques
Cutting and joining DNA molecules
Basic biology of plasmid and phage verctors
Cosmids, phasmids and other advanced vectors
Cloning strategies
Sequencing and mutagenesis
Cloning in bacteria other than Escherichia coli
Cloning in Saccharomyces cerevisiae and other fungi